Peptide-N4-(N-acetyl-β-glucosaminyl) asparagineamidase (PNGaseF)

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Name Glycopeptidase; PNGaseF
Product Code EN01022
E.C. 3.5.1.52
Product Description E. coli recombinant peptide-N4-(N-acetyl-β-glucosaminyl) asparagineamidase from Flavobacterium meningosepticum
Unit Definition One unit is defined as the amount of enzyme that catalyzes the release of 1 nmol N-glycan from RNaseB per minute at 37 °C.

Description

Peptide-N4-(N-acetyl-β-glucosaminyl) asparagineamidase (PNGaseF)

Accepted Name
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase.
Alternative Name(s)
Glycopeptidase.
Glycopeptide N-glycosidase.
N-glycanase.
N-oligosaccharide glycopeptidase.
Reaction catalysed
Hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl)asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue
Comment(s)
  • Does not act on (GlcNAc)-Asn, because it requires the presence of more than two amino-acid residues in the substrate (cf. EC 3.5.1.26).
  • Formerly EC 3.2.2.18.

Additional information

Pack

100 U, 250 U, 500 U, Other